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multianalyst software version 1 0 2  (Bio-Rad)


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    Bio-Rad multianalyst software version 1 0 2
    Multianalyst Software Version 1 0 2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multianalyst+software+version+1+0+2/ChromLab+Software/pmc05064770-75-18-22
    Average 96 stars, based on 3491 article reviews
    multianalyst software version 1 0 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Software:

    Article Title: A novel member of the glycosyltransferase family, beta 3 Gn-T2, highly downregulated in invasive human bladder transitional cell carcinomas.
    Article Snippet: Differential display reverse transcription (DDRT)±polymerase chain reaction (PCR) was used to compare the transcriptomes of invasive and noninvasive fresh human bladder transitional cell carcinomas.. A differentially expressed novel gene sharing structural similarity with the human b3-galactosyltransferase family, b-1,3-N-acetylglucosaminyltransferase-T2 (b3Gn-T2), was identified.. The full-length b3Gn-T2 cDNA, containing a complete open reading frame of 1193 bp, was cloned and sequenced. b3Gn-T2 exhibited 29±41% homology to the multigene b3-galactosyltransferase family.

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: Primary antibodies were labeled with goat antimouse IgG at a 1:10,000 dilution or with goat antirabbit IgG at a 1:4500 dilution conjugated with horseradish peroxidase (Dako, Hamburg, Germany) for 1 h. Immunoreactive bands were visualized by incubation of the membrane with Super Signal West Pico reagent from Pierce. .. Quanti®cation of the Bax/Bcl-2 ratio Quantitative analysis of the Bax/Bcl-2 protein expression was performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, Munich, Germany) as described previously (Prokop et al, 2000). ..

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. 226 10 Quantification of the prohibitin phosphorylation and steroidogenic protein ratios 227 Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression were 228 performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, 229 Munich, Germany) as described previously (Chowdhury et al., 2013). ..

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression was carried out using a scanning densitometer and Multianalyst Software version 1.0.2 (Bio-Rad) as described previously ( Chowdhury et al. 2013 ). ..

    Article Title: Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins
    Article Snippet: .. Quantitative analysis of the pro-apoptotic Bcl2 protein versus anti-apoptotic Bcl2 protein expression were performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, Munich, Germany) as described by Prokop et al. [ ]. .. Standardization of protein loading was achieved as follows: (1) protein measurements of all samples were performed using the Bio-Rad Protein assay kit and equal amounts of protein (25 μg per lane) were loaded on the gel; (2) transfer efficiency of the Western blots was routinely checked by staining the membranes with 0.5 % Ponceau Red in 1 % acetic acid; (3) for chemiluminescent detection, films were exposed for exactly the same length of time, and were optimized for each antibody used in this study; and (4) detection of Bax, Bak, Bclxl and Bcl2 were performed separately using the same membrane.

    Expressing:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: Primary antibodies were labeled with goat antimouse IgG at a 1:10,000 dilution or with goat antirabbit IgG at a 1:4500 dilution conjugated with horseradish peroxidase (Dako, Hamburg, Germany) for 1 h. Immunoreactive bands were visualized by incubation of the membrane with Super Signal West Pico reagent from Pierce. .. Quanti®cation of the Bax/Bcl-2 ratio Quantitative analysis of the Bax/Bcl-2 protein expression was performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, Munich, Germany) as described previously (Prokop et al, 2000). ..

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. 226 10 Quantification of the prohibitin phosphorylation and steroidogenic protein ratios 227 Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression were 228 performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, 229 Munich, Germany) as described previously (Chowdhury et al., 2013). ..

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression was carried out using a scanning densitometer and Multianalyst Software version 1.0.2 (Bio-Rad) as described previously ( Chowdhury et al. 2013 ). ..

    Article Title: Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins
    Article Snippet: .. Quantitative analysis of the pro-apoptotic Bcl2 protein versus anti-apoptotic Bcl2 protein expression were performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, Munich, Germany) as described by Prokop et al. [ ]. .. Standardization of protein loading was achieved as follows: (1) protein measurements of all samples were performed using the Bio-Rad Protein assay kit and equal amounts of protein (25 μg per lane) were loaded on the gel; (2) transfer efficiency of the Western blots was routinely checked by staining the membranes with 0.5 % Ponceau Red in 1 % acetic acid; (3) for chemiluminescent detection, films were exposed for exactly the same length of time, and were optimized for each antibody used in this study; and (4) detection of Bax, Bak, Bclxl and Bcl2 were performed separately using the same membrane.

    Phospho-proteomics:

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. 226 10 Quantification of the prohibitin phosphorylation and steroidogenic protein ratios 227 Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression were 228 performed using a scanning densitometer and Multianalyst Software Version 1.0.2 (Biorad, 229 Munich, Germany) as described previously (Chowdhury et al., 2013). ..

    Article Title: Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation
    Article Snippet: .. Quantitative analysis of the PHB phosphorylation and steroidogenic protein expression was carried out using a scanning densitometer and Multianalyst Software version 1.0.2 (Bio-Rad) as described previously ( Chowdhury et al. 2013 ). ..

    Control:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Western Blot:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Blocking Assay:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..

    Membrane:

    Article Title: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
    Article Snippet: .. [arbitrary units]b Bax/Bcl-2 ratioc MelHo ++ ++ 0.067 0.045 1.49 A375 +++ +++ 0.048 0.025 1.93 M186 + + 0.693 0.186 3.73 BRO ++ ++ 0.208 0.089 2.34 SkMel13 + + 0.518 0.062 8.36 M221 ± ± 0.047 0.058 0.82 SkMel23 (p53 mut.)d ± ± 0.071 0.396 0.18 MEWO (p53 mut.)d ± ± 0.030 0.201 0.15 Mel2A ± ± 0.034 0.096 0.36 SkMel28 ± ± 0.040 0.098 0.41 JPC298 ± ± 0.111 0.285 0.39 a+, low sensitivity (apoptotic cells 200% of control); ++, mean sensitivity (apoptotic cells 200%±300% of control); +++, high sensitivity (apoptotic cells 300%±600% of control) as measured by cell death detection ELISA bValues of Bax and Bcl-2 expression in different melanoma cell populations were quanti®ed by scanning densitometry of the Western blot shown in Fig 1 using the Multianalyst Software Version 1.0.2 (Biorad) and are given in arbitrary units. cA Bax/Bcl-2 ratio < 1.00 characterizes CD95-resistant cells and a Bax/Bcl-2 ratio > 1.00 CD95-sensitive melanoma cells. dp53 mut., p53 mutated. viability by blocking cytochrome c release and preventing loss of mitochondrial membrane potential (Yang et al, 1997). ..



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